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KMID : 0894520070110030187
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Development & Reproduction
2007 Volume.11 No. 3 p.187 ~ p.193
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Effect of Ethane 1,2-Dimethane Sulfonate(EDS) on the Expression of Steroid Hormone Receptors, 5α-reductase and Aromatase in the Rat Epididymis
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Son Hyeok-Jun
Lee Sung-Ho
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Abstract
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Ethane 1,2-dimethane sulfonate(EDS), a Leydig cell specific toxicant, has been widely used to create the reversible testosterone withdrawal rat model. Though the maintenance of epididymal structure and function is highly dependent on the testosterone secreted from testis, its derivatives, dihydroxytestosterone(DHT) and estrogen, might have crucial roles. The aim of present study was to monitor the expression patterns of sex steroid receptors, cytochrome P450 aromatase(P450arom) and 5α-reductase in the rat epididymis up to 7 weeks after EDS injection. Adult male rats(350～400 g) were injected with a single does of EDS(75 ㎎/㎏ i.p.) and sacrificed on weeks 0, 1, 2, 3, 4, 5, 6 and 7. The transcriptional activities of the target genes were evaluated by semi-quantitative RT-PCRs. The transcript level of estrogen receptor alpha(ERα) in EDS group was significantly higher than control level on week 1(P＜0.01). After week 2, there was no significant difference in ERα levels between EDS group and control. The transcript level of estrogen receptor beta(ERβ) in EDS group was significantly higher than control level on week 1(P＜0.05), lowered on weeks 2 and 3(P＜0.05 and P＜0.01, respectively), fluctuated during weeks 4 and 6, and elevated on week 7(P＜0.05). The androgen receptor (AR) message levels increased significantly week 2(P＜0.01), then returned to control level on week 3. In contrast, expression of cytochrome P450 aromatase(P450arom) decreased sharply during weeks 1～3(P＜0.01 on weeks 1 and 2; P＜0.05 on week 3), then went back to control level on week 4. The mRNA level of 5α-reductase type 2(5α-RT2) increased significantly on week 4(P＜0.01), then returned to control level. The present study indicated that EDS administration could induce reversible alterations in the transcriptional activities of sex steroid hormone receptors and androgenconverting enzymes in rat epididymis. EDS injection model will be useful to clarify the regulation mechanism of mammalian epididymal physiology.
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KEYWORD
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Ethane 1, 2-dimethane sulfonate(EDS), Sex steroid receptors, P450arom, 5α-reductase, Rat epididymis
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